Volume 50, Issue 2, Article 6
Molecular Typing of RhD-Negative Blood Donors With C and/or E Antigen
Guzijan et al. Scr Med 2019;50(2):97-101.
Gordana Guzijan1,2, Milanka Milosavić1, Danijela Radojković Sredić1, Marko Lilić3, Biljana Jukić1
(1) Institute for Transfusion Medicine of the Republic of Srpska, Banja Luka, Bosnia and Herzegovina.
(2) Faculty of Medicine, University of Banja Luka, the Republic of Srpska, Banja Luka, Bosnia and Herzegovina.
(3) Josip Juraj Strossmayer University of Osijek, Osijek, Croatia.
Background: Most people are either RhD positive or RhD negative, but there is also a number of persons with D antigen variants. The aim of this study was to prove, by using molecular diagnostic tests, whether the RHD gene and D antigen on the red cell membrane of the blood donors serologically typed as RhD-negative with RhD phenotype Ccddee and ccddEe, are so weak that they cannot be proven by serology techniques or the available anti-D test serums.
Methods: Samples used are those of regular voluntary donors who were serotyped as RhD-negative, C and/or E positive. Samples were collected from voluntary donors at the Institute for Transfusion Medicine of the Republic of Srpska during the period from April 2016 to December 2018.
Results: Among the serologically proven RhD-negative donors, 346 had C and/or E in their phenotype and those were subjected to molecular screening test.
Conclusion: The first results of molecular typing match those published in literature, i.e. the RHD gene is present in some serologically RhD-negative forms, which
was proven by molecular testing.
Key words: Rh system, D antigen, weak D, partial D, antigens, blood donors, phenotype.