ISSN 2490-3329 (Print)
ISSN 2303-7954 (Online)

Volume 50, Issue 1, Article 2

Djuric et al. Scr Med 2019;50(1):6-12.

ORIGINAL ARTICLE

The Effects of Gasotransmitters Inhibition on Homocysteine Acutely Induced Changes in Oxidative Stress Markers in Rat Plasma

Marko Djuric1, Sanja Kostic², Dragana Loncar-Stojiljkovic³, Slavica Mutavdzin4, Mirjana B. Colovic5, Danijela Krstic6, Predrag Stevanovic1 and Dragan M. Djuric4

(1) Department of Anesthesiology, Reanimatology and Intensive Care Medicine, University Clinical Hospital Center "Dr. Dragiša Mišović - Dedinje", Belgrade, Serbia
(2) Faculty of Medicine, University of Belgrade, Belgrade, Serbia
(3) Institute for Cardiovascular Diseases “Dedinje”, Belgrade, Serbia
(4) Institute of Medical Physiology “Richard Burian“, Faculty of Medicine, University of Belgrade, Belgrade, Serbia
(5) Department of Physical Chemistry, Vinča Institute of Nuclear Sciences, University of Belgrade, Belgrade, Serbia
(6) Institute of Chemistry in Medicine "Prof. Dr. Petar Matavulj", Faculty of Medicine, University of Belgrade, Belgrade, Serbia

Correspondence: DRAGAN M. DJURIC, E: This e-mail address is being protected from spambots. You need JavaScript enabled to view it , E: This e-mail address is being protected from spambots. You need JavaScript enabled to view it

 

DOI:10.5937/scriptamed50-21100

 

ABSTRACT

Background: The importance of homocysteine (Hcy) is increasingly recognized in last few decades as an independent risk factor for atherosclerosis and thrombosis, but there is lack of data referring to influence of Hcy on plasma oxidative stress parameters as well as the role of gasotransmitters in these effects. Therefore, this study aim was to assess the role of gasotransmitter inhibitors in Hcy-induced effects on plasma oxidative stress in rats.
Material and Methods: Study involved 96 male Wistar albino rats divided into 8 groups: 1) Control group – saline (1ml 0.9 % NaCl i.p.); 2) DL-Hcy (8 mmol/kg i.p. DL homocysteine (DL-Hcy); 3) L-NAME (10 mg/kg i.p. Nω-Nitro-L-arginine methyl ester (L-NAME), inhibitor of NO production); 4) ZnPPR IX (30 mol/kg i.p. protoporphyrin IX zinc (ZnPPR IX), inhibitor of CO production); 5) DL-PAG (50 mg//kg i.p. DL-propargylglycine (DL-PAG), inhibitor of H2S production); 6) DL-Hcy+L-NAME (8 mmol/kg i.p. DL-Hcy + 10 mg/kg i.p. L-NAME); 7) DL-Hcy+ZnPPR IX (8 mmol/kg i.p. DL-Hcy + 30 mol/kg i.p. Zn PPR IX), and 8) DL-Hcy+DL-PAG (8 mmol/kg i.p. DL-Hcy + 50 mg//kg i.p. DL-PAG). In all experimental groups, tested substances were administered in a single dose, intraperitoneally, 60 minutes before animals’ euthanasia. In the collected blood samples malondialdehyde concentration, catalase, glutathione peroxidase and superoxide dismutase activity were measured.
Results: Applied substances induced rapid and strong increase of plasma antioxidant enzymatic activity probably as a compensatory response to its pro-oxidant influence.
Conclusion: The effects of Hcy on the activity of plasma antioxidant enzymes are in part mediated via interaction with gasotransmitters.

Key words: gasotransmitters, homocysteine, oxidative stress markers, rat plasma.

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